PXD078164

PXD078164 is an original dataset announced via ProteomeXchange.

Title	Time-resolved integrative proteomic and metabolomic analysis of hypoxia-induced metabolic reshaping in vascular smooth muscle cells
Description	Hypoxia is a characteristic feature of the atherosclerotic plaque microenvironment and a critical driver of vascular remodeling. However, the dynamic transition of metabolic networks in vascular smooth muscle cells (VSMCs) remains incompletely understood. In this study, we performed a time-resolved analysis (0, 12, 24, and 48 h) of hypoxic VSMCs, utilizing integrated proteomics and metabolomics to map their temporal molecular landscape. Our data revealed a progressive impairment of mitochondrial oxidative phosphorylation, supported by a broad downregulation of oxidative pathway components and the validated suppression of key Complex I subunits. Conversely, central carbon metabolism underwent rapid and sustained upregulation at the protein level, marked by the induction of glucose transporter GLUT1 and key glycolytic enzymes including HK1/2, PFKFB3, GAPDH, ENO1, and PKM. Notably, while glycolytic enzyme levels increased consistently, their corresponding metabolic intermediates exhibited heterogeneous temporal fluctuations rather than simple accumulation. Furthermore, we identified a time-dependent shift in nitrogen and redox homeostasis, specifically the diversion of arginine metabolism toward the ornithine–polyamine pathway, alongside a gradual depletion of the reduced glutathione pool. Collectively, these findings demonstrate that hypoxia drives a highly coordinated and stage-specific metabolic adaptation in VSMCs, providing a systematic map of the metabolic reprogramming underlying vascular cell function in the hypoxic plaque microenvironment.
HostingRepository	iProX
AnnounceDate	2026-05-07
AnnouncementXML	Submission_2026-05-08_17:32:00.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yiwei Hu
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-08 17:31:34	ID requested
⏵ 1	2026-05-08 17:32:01	announced

Dataset with its publication pending

submitter keyword: Proteomics, Metabolomics, Hypoxia, Metabolic remodeling, Oxidative phosphorylation

Ling Lin
contact affiliation	Fudan University
contact email	linglin@fudan.edu.cn
lab head
Yiwei Hu
contact affiliation	Fudan University
contact email	huyiwei78@126.com
dataset submitter

iProX dataset URI