PXD078120

PXD078120 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Intact Protein TMT Labeling and 2D High-pH/Low-pH RP/RPLC-MS/MS
Description	Optimization of intact protein TMT labeling and multidimensional high-pH/low-pH RP/RPLC-MS/MS. Application of this platform for quantification of proteoform-level abundance changes as a result of staursporine treatment in HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_04:06:26.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kellye Sutton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-07 08:26:23	ID requested
⏵ 1	2026-08-13 04:06:27	announced

Publication List

10.1016/j.aca.2026.345846;
Guo Y, Chowdhury T, Galie WP, Cupp-Sutton KA, Zhao Z, Solenberger A, Yu D, Wu S, Deep intact proteoform quantification of low-microgram-scale proteome by tandem mass tag labeling and online 2D high-pH/low-pH RPLC top-down analysis. Anal Chim Acta, 1418():345846(2026) [pubmed]

10.1016/j.aca.2026.345846;

Guo Y, Chowdhury T, Galie WP, Cupp-Sutton KA, Zhao Z, Solenberger A, Yu D, Wu S, Deep intact proteoform quantification of low-microgram-scale proteome by tandem mass tag labeling and online 2D high-pH/low-pH RPLC top-down analysis. Anal Chim Acta, 1418():345846(2026) [pubmed]

Keyword List

submitter keyword: TMT labeling,Top-down proteomics, multidimensional LC-MS

submitter keyword: TMT labeling,Top-down proteomics, multidimensional LC-MS

Contact List

Si Wu
contact affiliation	The University of Alabama
contact email	swu49@ua.edu
lab head
Kellye Sutton
contact affiliation	University of Alabama
contact email	kasutton3@ua.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078120
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078120

PRIDE project URI

Repository Record List

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