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PXD078087
PXD078087 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoenolpyruvate carboxykinase controls Edwardsiella tarda virulence via oxaloacetate |
| Description | Understanding the metabolic basis of bacterial virulence is essential for developing anti-infective strategies. Phosphoenolpyruvate carboxykinase (PEPCK), encoded by pckA, is a key enzyme at the metabolic junction between the pyruvate cycle and the TCA cycle, but its role in virulence regulation remains largely unexplored. In this study, we generated a pckA deletion mutant, ΔpckA, in Edwardsiella tarda and investigated its impact on virulence, metabolism, and vaccine potential. The ΔpckA mutant exhibited increased antibiotic sensitivity, faster growth, reduced autoaggregation, and significantly attenuated virulence in a tilapia infection model. Quantitative proteomics revealed global downregulation of type III and type VI secretion systems (T3SS and T6SS) and upregulation of flagellar proteins. Mechanistically, accumulation of oxaloacetate (OAA) in the mutant suppressed T3SS/T6SS expression without affecting flagellar motility. Importantly, ΔpckA induced a robust immune response in zebrafish and conferred strong protection against lethal challenge, with relative percent survival values of 64% and 81.82% in zebrafish and tilapia, respectively. Taken together, our findings identify PEPCK as a critical metabolic regulator of E. tarda virulence and highlight ΔpckA as a promising live attenuated vaccine candidate. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-06 |
| AnnouncementXML | Submission_2026-05-06_17:55:00.559.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiayao Wu |
| SpeciesList | scientific name: Edwardsiella tarda EIB202; NCBI TaxID: 498217; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-06 17:54:45 | ID requested | |
| ⏵ 1 | 2026-05-06 17:55:01 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: phosphoenolpyruvate carboxykinase, oxaloacetate, virulence, Edwardsiella tarda, auto-aggregation |
Contact List
| Bo Peng | |
|---|---|
| contact affiliation | Sun Yat-sen University |
| contact email | pengb26@mail.sysu.edu.cn |
| lab head | |
| Jiayao Wu | |
| contact affiliation | Sun Yat-sen University |
| contact email | wujy365@mail2.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
