PXD078078

PXD078078 is an original dataset announced via ProteomeXchange.

Title	A selectivity filter in Tim17 mediates intramitochondrial protein sorting - Dataset III
Description	Mitochondria import hundreds of proteins into the matrix and the inner membrane. The translocase of the inner membrane of mitochondria (TIM23 complex) sorts these proteins using topogenic signals in their structure and directs them into the matrix or inserts them into the inner membrane. How these signals are deciphered is unknown. Here we show that Tim17, the protein-conducting subunit of the TIM23 complex, contains a highly conserved selection filter for incoming proteins. Using a fluorescence-based sorting reporter, we identified the novel mitochondrial oxidoreductase Dmo2 (akin to DMAC1 in humans) as critical factor for intramitochondrial protein sorting. Molecular dynamics simulations show that a Dmo2-dependent disulfide bond into Tim17 constitutes a negatively charged selection filter at the vestibule of the TIM23 complex that arrests membrane proteins and facilitates the lateral insertion. Our work provides a first molecular rationale as to how the TIM23 translocase differentiates between soluble and membrane proteins.
HostingRepository	PRIDE
AnnounceDate	2026-08-03
AnnouncementXML	Submission_2026-08-03_07:09:24.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-06 13:58:00	ID requested
⏵ 1	2026-08-03 07:09:25	announced

Dataset with its publication pending

submitter keyword: None

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern, Germany
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078078
     1. Label: PRIDE project
     2. Name: A selectivity filter in Tim17 mediates intramitochondrial protein sorting - Dataset III