PXD078069

PXD078069 is an original dataset announced via ProteomeXchange.

Title	HPLC-MS/MS analysis of the S-monooxygenated S-Trp (O=S-Trp) bearing peptide in heme reconstituted EcKatG S-Trp105
Description	A sulfur-containing analog of tryptophan, thiotryptophan (3-benzothienyl-L-alanine, S-Trp), was genetically incorporated into EcKatG to replace the MYW-forming tryptophan, W105. The heme free apo-protein was purified, after heme-reconstitution and peroxide treatment, the S-Trp-bearing peptide was isolated using trypsin digestion followed by HPLC separation. Tandem mass spectrometry (MS/MS) was then performed to verify the substitution. The final results demonstrated that S-Trp was successfully incorporated and subsequently underwent S-monooxygenation, yielding O=S-Trp.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_08:49:09.766.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078069
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ran Duan Duan
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-06 06:20:35	ID requested
⏵ 1	2026-05-13 08:49:10	announced

10.6019/PXD078069;

submitter keyword: catalase-peroxidase, Non-canical amino acid,KatG

Aimin Liu
contact affiliation	Professor Emeritus Department of Chemistry The University of Texas at San Antonio Effective July 1, 2026: Johnson Foundation Professor of Chemical Enzymology Department of Biochemistry and Biophysics, Perelman School of Medicine Department of Chemistry, School of Arts and Sciences University of Pennsylvania, Philadelphia, PA 19104
contact email	aimin.liu@utsa.edu
lab head
Ran Duan Duan
contact affiliation	University of Texas at San Antonio
contact email	ran.duan@utsa.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078069
     1. Label: PRIDE project
     2. Name: HPLC-MS/MS analysis of the S-monooxygenated S-Trp (O=S-Trp) bearing peptide in heme reconstituted EcKatG S-Trp105