PXD078061

PXD078061 is an original dataset announced via ProteomeXchange.

Title	Cd-IMAC-MS profiles cadmium-associated proteomics in cadmium-exposed rat brain
Description	Cadmium (Cd2+) is a persistent neurotoxic trace metal, yet the native protein targets and protein assemblies associated with cadmium exposure in brain tissue remain incompletely defined. Here, we developed Cd-IMAC-MS, a non-denaturing workflow that couples Cd2+-charged nitrilotriacetic acid (NTA) affinity enrichment with data-independent acquisition (DIA) proteomics to profile cadmium-associated proteins from rat brain. To distinguish Cd-dependent enrichment from bead-derived background and nonspecific adsorption, the discovery experiment used a 2 × 2 design and double-ratio correction (CH/CL versus BH/BL). Cd-NTA enrichment quantified 2,989 proteins and 37,947 peptides, whereas blank-NTA controls quantified 2,425 proteins and 20,559 peptides. The correction framework resolved 80 high-confidence cadmium-associated candidates, including 57 proteins quantified across both matrices and 23 proteins detected only in Cd-NTA enrichments. STRING and cytoHubba analyses identified ANXA5, ANXA2, TOP2A, and SQSTM1 as consensus network hubs. An independent cadmium exposure cohort further prioritized eight reproducible candidates—CTBP1, IQSEC3, TAGLN2, ANXA2, ANXA1, NUCB1, PDHA1, and USP15—linking cadmium exposure to calcium-responsive membrane organization, mitochondrial metabolism, vesicle trafficking, proteostasis, and inflammatory or stress signaling. UniProt annotation and MIB2 residue-level prediction further indicated metal-binding features in CTBP1, IQSEC3, TAGLN2, and USP15. Collectively, Cd-IMAC-MS provides a scalable strategy for native cadmium-associated protein discovery in complex brain tissue and yields a prioritized resource for mechanistic and translational studies of cadmium neurotoxicity.
HostingRepository	iProX
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-06_02:18:54.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaoxin Huang
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-06 02:18:35	ID requested
⏵ 1	2026-05-06 02:18:55	announced

Dataset with its publication pending

submitter keyword: Cd-IMAC-MS, cadmium-associated proteins, cadmium neurotoxicity, DIA proteomics

Shaoxin Huang
contact affiliation	Jiujiang University
contact email	huangshaoxinok@163.com
lab head
Shaoxin Huang
contact affiliation	Jiujiang University
contact email	huangshaoxinok@163.com
dataset submitter

iProX dataset URI