PXD078047

PXD078047 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of 3T3 murine fibroblast used as feeder-layer
Description	The unfolded protein response (UPR) helps reinstate cellular proteostasis upon an accumulation of misfolded proteins in the endoplasmic reticulum (ER), in part through ER-associated degradation (ERAD). Ube2j2 is an ER-localized E2 ubiquitin-conjugating enzyme that participates in ERAD. We used mass spectrometry analysis of cultured U2OS cells to investigate how the loss of Ube2j2 affects the cellular proteome in response to tunicamycin-induced ER stress. We constructed a network of twelve statistically distinct modules of protein abundance profiles across conditions. We describe the Gene Ontology annotations for each module along with the “hub gene” proteins whose abundance levels most closely adhere to each module’s protein abundance profile. Our analysis identifies known Ube2j2-associated pathways (e.g., the UPR and ERAD) and cellular functions that were previously unassociated with Ube2j2 (e.g., RNA metabolism, ER-Golgi transport, and cell-cycle progression). These data provide avenues for further investigation into the cellular functions of Ube2j2 under basal and ER-stressed conditions.
HostingRepository	PRIDE
AnnounceDate	2026-06-03
AnnouncementXML	Submission_2026-06-03_04:47:14.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Marino
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	mono N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-06 01:36:52	ID requested
⏵ 1	2026-06-03 04:47:14	announced

Dataset with its publication pending

submitter keyword: feeder-layer,fibroblast, skin expansion

Elena Enzo
contact affiliation	Centre for Regenerative Medicine “Stefano Ferrari”, University of Modena and Reggio Emilia, Modena, Italy
contact email	elena.enzo@unimore.it
lab head
Antonio Marino
contact affiliation	Proteomics Research Infrastructure (PRI) - UCPH
contact email	antonio.marino@pri.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD078047

PRIDE project URI

• PRIDE
  1. PXD078047
     1. Label: PRIDE project
     2. Name: Proteomic analysis of 3T3 murine fibroblast used as feeder-layer