PXD078020

PXD078020 is an original dataset announced via ProteomeXchange.

Title	Identification of novel HLA class I-restricted hepatitis B virus peptides and their modulation by peptide editor TAPBPR
Description	Hepatitis B virus (HBV) is the leading cause of hepatocellular carcinoma and infections remain a substantial global health problem for which no curative therapy exists. As cytotoxic CD8+ T lymphocytes are the principal effectors of natural HBV resolution, there is considerable interest in discovering novel HBV epitopes that are presented for immune recognition. Current knowledge of HBV-derived peptide presentation to CD8+ T cells is largely dominated human leukocyte antigen (HLA)-A*02:01. Our aim was to characterise novel HBV-derived peptides presented on HLA-B and -C molecules, particularly allotypes that are prevalent in regions with high HBV infection rates. Additionally, we sought to determine if the recently discovered major histocompatibility complex class I (MHC-I) peptide editor TAPBPR modulated expression of HBV-derived epitopes. To discover new HBV-derived epitopes, immunopeptidomic analysis was performed on cells expressing single HLA class I molecules transduced to express individual HBV proteins. Peptide binding assays were used to validate HLA binding of selected peptides. To determine the effect of TAPBPR on the HBV peptidome, immunopeptidomic analysis was performed on either wild-type HeLa cells expressing HBV proteins or their TAPBPRKO counterpart. The results were validated using TAPBPR peptide-exchange assays. We identified 28 novel HBV-derived peptides presented on HLA class I. Importantly, 22 of the newly discovered HBV-derived peptides were identified on cells expressing HLA-B*08:01, -B*15:03, -B*35:01, -C*06:02 and -C*12:03. Additionally, TAPBPRKO HeLa cells presented four additional and novel HBV-derived peptides. This work presents a substantial expansion of the HLA-B and HLA-C-restricted HBV-derived peptides described in the Immune Epitope Database (IEDB). Additionally, this work represents the first indication that TAPBPR shapes not only the cellular but also the viral peptide repertoire presented on MHC-I molecules.
HostingRepository	PRIDE
AnnounceDate	2026-07-15
AnnouncementXML	Submission_2026-07-15_05:53:14.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Bauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-05 11:40:50	ID requested
⏵ 1	2026-07-15 05:53:14	announced

Dataset with its publication pending

submitter keyword: Immunopeptidomics, HLA, TAPBPR, MHC

Juliane Sarah Walz
contact affiliation	Department of Peptide-based Immunotherapy, Institute of Immunology, University and University Hospital Tübingen, Tübingen, Germany
contact email	juliane.walz@med.uni-tuebingen.de
lab head
Jens Bauer
contact affiliation	Clinical Collaboration Unit Translational Immunology, German Cancer Consortium (DKTK), Department of Internal Medicine, University Hospital Tübingen, Tübingen, Germany
contact email	j.bauer@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078020
     1. Label: PRIDE project
     2. Name: Identification of novel HLA class I-restricted hepatitis B virus peptides and their modulation by peptide editor TAPBPR