PXD078004

PXD078004 is an original dataset announced via ProteomeXchange.

Title	Mechanism of spliceosome termination
Description	After removing an intron from pre-mRNA, the spliceosome becomes trapped in a non productive complex bound to the excised intron. The termination of this complex is critical for spliceosome recycling and intron decay, but the underlying mechanism remains unknown. Here, we present cryo-electron microscopy structures of human spliceosomes captured during two sequential stages of termination. First, the RNA helicases DHX15 and Aquarius unwind the spliceosome’s RNA active site, releasing associated factors including U2 snRNA, and extracting the buried intron-lariat branch point. The extracted Branch point is then debranched by the spliceosome-tethered debranching enzyme DBR1. This generates the debranched intron spliceosome, which recruits the RNA helicase DHX35 via co-factors GPATCH1–WDR83 and YJU2B. DHX35 then ejects the debranched intrón from the U6 snRNA–5′ splice site duplex, driving spliceosome disassembly and intrón turnover. In defective spliceosomes stalled on aberrant introns, YJU2B collaborates with a different protein partner, LENG1, to recruit DHX35–GPATCH1–WDR83 to terminate these defective complexes through spliceosomal quality control. Taken together, we reveal shared but distinct mechanisms governing regular and defective spliceosome termination,ensuring accurate and efficient pre-mRNA splicing.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_13:45:19.931.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-05 05:45:52	ID requested
⏵ 1	2026-08-17 13:45:20	announced

Dataset with its publication pending

submitter keyword: spliceosome

Román González-Prieto
contact affiliation	CABIMER
contact email	roman.gonzalez@cabimer.es
lab head
Román González-Prieto
contact affiliation	Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact email	roman.gonzalez@cabimer.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078004

PRIDE project URI

• PRIDE
  1. PXD078004
     1. Label: PRIDE project
     2. Name: Mechanism of spliceosome termination