PXD077896

PXD077896 is an original dataset announced via ProteomeXchange.

Title	A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions
Description	Virological research has traditionally focused on individual viruses or viral families. Advances in DNA synthesis now allow large-scale construction of individual gene products, enabling systematic exploration of the virome. Here, we developed a barcoded library of ~12,000 viral open reading frames (vORFs) from 513 viral species, which we leveraged to agnostically identify regulators of cellular proliferation and the innate and adaptive immune responses. Using this platform, we identified hundreds of viral regulators of cellular proliferation, class I MHC antigen presentation, and interferon signaling. Integrating results across these screens revealed unique phenotypic profiles and functional vORF modules, allowing the in-depth characterization of two previously uncharacterized viral proteins, MC162R and YLDV 151R, which impair class I MHC antigen presentation and IFN signaling, respectively. Together, the viral ORFeome provides a scalable framework for dissecting viral protein function across the breadth of the virome.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:39.814.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD077896
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Colin O'Leary
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-30 18:57:20	ID requested
⏵ 1	2026-09-07 04:21:40	announced

10.1016/j.cell.2026.05.024;

10.6019/PXD077896;

Fujimura E, O'Leary CN, Li MZ, Roberts RA, Glassman CR, Paulo JA, Jiang H, Abdelfattah NS, Wooten EC, Mirman Z, Harper JW, Cole PA, Elledge SJ, A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions. Cell, 189(17):5411-5428.e13(2026) [pubmed]

submitter keyword: MC162R, virome

Stephen Elledge
contact affiliation	Department of Genetics, Harvard Medical School Division of Genetics, Brigham and Women's Hospital Howard Hughes Medical Institute
contact email	selledge@genetics.med.harvard.edu
lab head
Colin O'Leary
contact affiliation	Division of Genetics, Brigham & Women's Hospital Department of Genetics, Harvard Medical School
contact email	cno487@g.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077896

PRIDE project URI

• PRIDE
  1. PXD077896
     1. Label: PRIDE project
     2. Name: A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions