PXD077837

PXD077837 is an original dataset announced via ProteomeXchange.

Title	LRRC71 is essential for sperm motility, fertilization and male fertility
Description	Although sperm supply half the genomic material necessary for generating viable offspring in sexual reproduction, the core molecular mechanisms underlying their formation remain largely unelucidated. Here, we demonstrate that leucine-rich repeat-containing 71 (LRRC71) is essential for spermatogenesis and male fertility in mice. Lrrc71 knockout (KO) mice exhibit asthenozoospermia and disrupted mitochondrial sheaths in sperm. Both glycolysis and oxidative phosphorylation (OXPHOS) pathways were disrupted in Lrrc71 KO sperm, resulting in reduced ATP content. Furthermore, Lrrc71 KO sperm showed defective fertilization capacity and failed sperm migration into the oviduct. Key metabolic enzymes and fertilization-related proteins were significantly down-regulated in Lrrc71 KO sperm, and co-immunoprecipitation validated their interactions with LRRC71. Also, a novel heterozygous variant of LRRC71 was identified in a patient with asthenozoospermia. Despite failed in vitro fertilization (IVF), a successful intracytoplasmic sperm injection outcome was achieved in Lrrc71 KO mice. These findings demonstrate the essential role of LRRC71 in regulating spermatogenesis and sperm function, which facilitates our understanding of molecular mechanisms underlying spermatogenesis. LRRC71 represents a potential target for the diagnosis and treatment of male infertility associated with asthenozoospermia.
HostingRepository	iProX
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-06-08_02:19:39.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lu Yuan
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-29 20:44:48	ID requested
1	2026-04-29 20:45:10	announced
⏵ 2	2026-06-08 02:19:46	announced	2026-06-08: Add info

Yuan L, Xu C, Ge T, Li G, Meng S, Lu W, Yang Y, Zhao Y, Fang X, Zhao Y, Niu C, Zeng X, Yang F, Zheng Y, LRRC71 is essential for sperm motility, fertilization and male fertility. J Biol Chem, ():113176(2026) [pubmed]

submitter keyword: LRRC71, male infertility, asthenozoospermia, sperm motility,energy metabolism, fertilization

Ying Zheng
contact affiliation	Yangzhou University
contact email	yzzkl@163.com
lab head
Lu Yuan
contact affiliation	Yangzhou University
contact email	yuanlu0217@163.com
dataset submitter

iProX dataset URI