PXD077818 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | EZH2 pre-mRNA pull-down screening for proteins that regulate EZH2 alternative splicing |
| Description | Post-transcriptional regulation mediated by RNA-binding proteins is essential for B cell development and differentiation. EZH2 is a key regulatory protein involved in B cell development and the germinal center response, and its expression is subject to alternative splicing regulation. Skipping of exon 14 in EZH2 disrupts its catalytic activity for H3K27me3 deposition. To identify proteins that regulate EZH2 alternative splicing in B cells, we performed an RNA pull-down assay using exon 14 together with part of intron 14 of EZH2 pre-mRNA, followed by mass spectrometry analysis of the pulled-down proteins. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-30 |
| AnnouncementXML | Submission_2026-04-29_19:05:26.515.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhijian Zhu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-29 15:05:49 | ID requested | |
| ⏵ 1 | 2026-04-29 19:05:27 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Pull down,EZH2 |
Contact List
| Xijun Ou |
|---|
| contact affiliation | southern university of science and technology |
| contact email | ouxj@sustech.edu.cn |
| lab head | |
| Zhijian Zhu |
|---|
| contact affiliation | southern university of science and technology |
| contact email | zhuzhijian2017@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD077818 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077818
- Label: PRIDE project
- Name: EZH2 pre-mRNA pull-down screening for proteins that regulate EZH2 alternative splicing
