PXD077768

PXD077768 is an original dataset announced via ProteomeXchange.

Title	A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions
Description	Virological research has traditionally focused on individual viruses or viral families. Advances in DNA synthesis now allow large-scale construction of individual gene products, enabling systematic exploration of the virome. Here, we developed a barcoded library of ~12,000 viral open reading frames (vORFs) from 513 viral species, which we leveraged to agnostically identify regulators of cellular proliferation and the innate and adaptive immune responses. Using this platform, we identified hundreds of viral regulators of cellular proliferation, class I MHC antigen presentation, and interferon signaling. Integrating results across these screens revealed unique phenotypic profiles and functional vORF modules, allowing the in-depth characterization of two previously uncharacterized viral proteins, MC162R and YLDV 151R, which impair class I MHC antigen presentation and IFN signaling, respectively. Together, the viral ORFeome provides a scalable framework for dissecting viral protein function across the breadth of the virome.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:21:39.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-28 18:45:58	ID requested
⏵ 1	2026-09-07 04:21:39	announced

10.1016/j.cell.2026.05.024;

submitter keyword: virome, MC162R, YLDV 151R, TMT, MHC

Stephen J. Elledge
contact affiliation	Department of Genetics, Harvard Medical School, Boston, MA 021152 Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA0211 Department of Cell Biology, Harvard Medical School, Boston, MA 02115 Howard Hughes Medical Institute, Boston, MA 02115
contact email	selledge@genetics.med.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077768

PRIDE project URI

• PRIDE
  1. PXD077768
     1. Label: PRIDE project
     2. Name: A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions