PXD077759

PXD077759 is an original dataset announced via ProteomeXchange.

Title	Serum Proteomics Analysis Identified Junction Plakoglobin and Glyceraldehyde-3-Phosphate Dehydrogenase as Potential Novel Circulating Biomarkers for Patients with Multiple Sclerosis
Description	Multiple Sclerosis (MS) is a multifaceted neurological condition associated with recurrent inflammatory episodes, demyelination, axonal injury, and resultant functional impairment. MS has a diverse array of clinical manifestations, with the progressive variants culminating in irreparable neurological impairment. Sensitive and accurate biomarkers are required to diagnose, predict disease progression, and tailor therapy methods for MS. Here, we utilized liquid chromatography mass spectrometry-based Proteomics (LC-MS/MS) to find biomarkers in serum from patients with MS. The study examined variations of protein expression in healthy (n = 20) and MS groups (n = 60), comprising 20 patients in each disease course (relapsing-remitting (RRMS), primary progressive (PPMS), and secondary progressive MS (SPMS)). The enzyme-linked immune assay (ELISA) was then used to verify a candidate protein on serum samples obtained from patients with MS (n = 15) and healthy controls (HC) (n = 18) (validation cohort). Our findings showed 12 differentially expressed proteins (DEPs). Among the discovered proteins, some have been previously documented in patients with MS and animal models, while others constitute novel findings. We found two proteins, junction plakoglobin protein (JUP) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), that were downregulated and upregulated in patients with MS in comparison with HCs, respectively. The validation results of these two proteins by ELISA were consistent with those from LC-MS/MS. The identification of these proteins may assist in the elucidation of pathogenetic processes and might open the door to novel methods of therapy for individuals with MS.
HostingRepository	PRIDE
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-04-28_17:00:49.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Core Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-28 14:45:20	ID requested
⏵ 1	2026-04-28 17:00:50	announced

Dataset with its publication pending

submitter keyword: Multiple Sclerosis, Serum, Proteomics

Prof. Mohammad Harb Semreen
contact affiliation	1. Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates. 2. College of Pharmacy, Department of Medicinal Chemistry, University of Sharjah, 27272, United Arab Emirates
contact email	msemreen@sharjah.ac.ae
lab head
Core Facility
contact affiliation	Sharjah Institute for Medical Research, University of Sharjah
contact email	tims-tof@sharjah.ac.ae
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD077759

PRIDE project URI

• PRIDE
  1. PXD077759
     1. Label: PRIDE project
     2. Name: Serum Proteomics Analysis Identified Junction Plakoglobin and Glyceraldehyde-3-Phosphate Dehydrogenase as Potential Novel Circulating Biomarkers for Patients with Multiple Sclerosis