PXD077754

PXD077754 is an original dataset announced via ProteomeXchange.

Title	Metabolic enzymes of human SGBS adipocytes interact with halogenated bisphenol A alternatives – TPP Data
Description	This dataset contains mass spectrometry–based thermal proteome profiling data. The assay was performed using lysates from differentiated human SGBS adipocytes. Cells were lysed, and protein concentrations were normalized prior to incubation with bisphenols, rosiglitazone, or vehicle control, followed by application of a temperature gradient to assess compound-induced changes in protein thermal stability. Soluble protein fractions were processed using SP3 bead–based cleanup, reduced, alkylated, and digested with trypsin. Peptides were labeled with tandem mass tags (TMT) to enable multiplexed quantitative analysis. Mass spectrometry data were analyzed using the R package TPP, including normalization, melting curve fitting, and determination of protein melting temperatures. Compound-induced thermal stability shifts were identified based on statistically significant melting point differences and stringent quality control criteria to ensure reproducibility and reliability of candidate targets.
HostingRepository	PRIDE
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_04:06:31.162.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD077754
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jasmin Kleißen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-28 13:34:36	ID requested
⏵ 1	2026-08-13 04:06:31	announced

10.6019/PXD077754;

10.1016/J.JHAZMAT.2026.143238;

ProteomeXchange project tag: Toxicoproteomics (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: thermal proteome profiling,BPA, halogenated BPA alternatives, TCA cycle, steroid metabolism., adipocytes, metabolic disruption

Kristin Schubert
contact affiliation	Previous: Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany Current: Cell Biology, Faculty of Environment and Natural Science, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany
contact email	kristin.schubert@b-tu.de
lab head
Jasmin Kleißen
contact affiliation	UFZ Leipzig
contact email	jasmin.kleissen@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD077754

PRIDE project URI

• PRIDE
  1. PXD077754
     1. Label: PRIDE project
     2. Name: Metabolic enzymes of human SGBS adipocytes interact with halogenated bisphenol A alternatives – TPP Data