⮝ Full datasets listing
PXD077718
PXD077718 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | High-Efficiency Cross-Linker TDS Enables Low-Input In Vivo Structural and Interactomic Analysis |
| Description | In vivo chemical cross-linking mass spectrometry (XL-MS) is powerful for capturing protein-protein interactions and dynamics in native contexts, but its high sample requirement (typically E7–E8 cells) hinders application to rare cell populations. To overcome this, we developed a novel low-input in vivo XL-MS strategy. This approach synergizes a miniaturized, transfer-free one-pot workflow with a custom-designed cross-linker, trehalose disuccinimidyl ester (TDS). TDS is highly biocompatible and membrane-permeable, enabling efficient in vivo cross-linking with minimal cellular perturbation. Crucially, TDS is MS-cleavable; upon fragmentation, it generates diagnostic reporter ions that linearize the data retrieval process, dramatically simplifying identification and boosting sensitivity compared to the quadratic complexity of analyzing non-cleavable cross-linkers. Furthermore, its inherent MS-compatibility allows it to remain in the sample throughout processing without interference, eliminating cleanup steps that cause sample loss. This integrated strategy robustly captures interactome and structural information from as few as 1,000 HeLa cells, identifying a network of 273 proteins connected by 1,055 unique cross-links, and extends the detection limit to 100 cells. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-06-28 |
| AnnouncementXML | Submission_2026-06-29_08:00:04.154.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yu Xia |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | 4800 Proteomics Analyzer |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-28 01:57:39 | ID requested | |
| ⏵ 1 | 2026-06-29 08:00:04 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: XL-MS |
Contact List
| Beirong Zhang;Yu Xia;Yi Liu | |
|---|---|
| lab head | |
| Yu Xia | |
| contact affiliation | 大连化学物理研究所 |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004579/ |
