PXD077705

PXD077705 is an original dataset announced via ProteomeXchange.

Title	Raw mass spectrometry data for the identification of GmGNAT10 as a key acetyltransferase mediating acetylation modification of Rubisco in soybean
Description	Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the core rate-limiting enzyme of photosynthetic carbon metabolism, is regulated by acetylation, yet its upstream acetyltransferases remain largely uncharacterized. Here, we identified a novel soybean acetyltransferase GmGNAT10, which interacts with Rubisco and other photosynthetic enzymes via IP-MS/MS interactomics. Quantitative acetylomics under fluctuating light revealed dramatic genotypic differences in acetylation levels of key RbcL lysine residues (K23, K175, K236) between wild-type and GmGNAT10 mutants. Our findings expand the mechanistic understanding of chloroplast non-histone acetylation in fine-tuning photosynthetic carbon metabolism.
HostingRepository	iProX
AnnounceDate	2026-04-28
AnnouncementXML	Submission_2026-04-27_23:41:39.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunmei Luo
SpeciesList	scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-27 23:41:03	ID requested
⏵ 1	2026-04-27 23:41:39	announced

Dataset with its publication pending

submitter keyword: Acetyltransferase, Rubisco, Soybean, Chloroplast, Acetylome, IP-MS

Mingnan Qu
contact affiliation	College of Agriculture
contact email	qmn@yzu.edu.cn
lab head
Chunmei Luo
contact affiliation	Yangzhou University
contact email	18605861790@163.com
dataset submitter

iProX dataset URI