⮝ Full datasets listing
PXD077668
PXD077668 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Affinity chromatography with ConBr lectin (Canavalia brasiliensis) for glycoprotein purification and characterization from COVID-19 patient serum |
| Description | Serum samples from COVID-19 patients (severe, mild, symptomatic non-COVID, and negative controls) were subjected to albumin depletion and affinity chromatography using ConBr lectin (Canavalia brasiliensis) coupled to sepharose matrix and a commercial ConA matrix (HiTrap ConA Sepharose 4B, Canavalia ensiformis), both specific for glucose/mannose. Eluted fractions were analyzed by nano-LC-MS/MS on an Orbitrap Velos system (Easy nLC1000, EASY-Spray PepMap C18 50cm x 75um, 300 nL/min). Data-dependent acquisition (DDA, top-20) with label-free quantification (LFQ) was performed. Database search used Sequest HT in Proteome Discoverer v3.1 against SwissProt Homo sapiens (TaxID:9606) and SARS-CoV-2 (UP000464024), with Carbamidomethyl (C) as fixed modification and Oxidation (M) as dynamic modification. Ethics approval: CEP CEUMA No. 209/2021-ESP/MA. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-27_12:19:20.690.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Camilo Torres |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-27 12:13:23 | ID requested | |
| ⏵ 1 | 2026-04-27 12:19:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: ConBr, SARS-CoV-2, Label-free quantification, Immune response, DatasetType:Proteomics |
Contact List
| Luis Claudio Nascimento da Silva | |
|---|---|
| contact affiliation | CEUMA |
| contact email | luiscn.silva@ceuma.br |
| lab head | |
| Camilo Torres | |
| contact affiliation | CEUMA |
| contact email | jctorresrom@unal.edu.co |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101621/ |
