⮝ Full datasets listing
PXD077658
PXD077658 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Idiopathic Nephrotic Syndrome Differentially Shapes the Proteome of Urinary Extracellular Vesicles |
| Description | This dataset contains label-free LC-MS/MS proteomic profiles of urinary extracellular vesicles (uEVs) isolated from pediatric patients affected by idiopathic nephrotic syndrome (INS). Patients were classified into steroid-sensitive (SSNS), steroid-dependent (SDNS), and steroid-resistant (SRNS) subgroups based on clinical response to corticosteroid therapy. To minimize confounding effects of proteinuria, only samples collected during remission were included. uEVs were isolated from second-morning urine samples using ultracentrifugation following HKUPP guidelines, with ZnSO4 treatment to reduce Tamm Horsfall protein contamination. Proteins were processed using a filter-aided sample preparation (FASP) protocol and analyzed by nanoLC-MS/MS on a Bruker Impact HD system in data-dependent acquisition (DDA) mode. The dataset includes technical duplicate injections per sample. Samples are labeled according to Patient ID (EXO SN 0XX), to clinical subgroup as S (SSNS), D (SDNS), and R (SRNS), and identifiers encode both subgroup ("gruppo"/"group") and replicate information ("injX"). Label-free quantification was performed using PEAKS Studio, and protein identification was conducted against the UniProt human database using stringent filtering criteria (at least 2 unique peptides). Overall, this dataset supports the identification of subgroup-specific proteomic signatures associated with INS clinical phenotypes and provides a resource for investigating extracellular vesicle-mediated mechanisms in kidney disease. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-29 |
| AnnouncementXML | Submission_2026-04-29_01:30:20.731.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Clizia Chinello |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | impact HD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-27 06:03:50 | ID requested | |
| ⏵ 1 | 2026-04-29 01:30:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: urinary extracellular vesicles, idiopathic nephrotic syndrome, pediatric nephrology, kidney disease, proteomics, LC-MS/MS, label-free quantification, steroid resistance, DatasetType:Proteomics |
Contact List
| Lucia Santorelli | |
|---|---|
| contact affiliation | University of Milano-Bicocca |
| contact email | l.santorelli@campus.unimib.it |
| lab head | |
| Clizia Chinello | |
| contact affiliation | University of Milano Bicocca |
| contact email | clizia.chinello@unimib.it |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101611/ |
