⮝ Full datasets listing
PXD077596
PXD077596 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Targeted Proteomics for Quantitation of Pseudomonas aeruginosa Podovirus LUZ19 |
| Description | This dataset supports a study describing the first validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for quantitation of a therapeutic bacteriophage. Bacteriophage therapy is an emerging treatment strategy for drug-resistant bacterial infections, yet the analytical methods available for quantifying phages in biological samples have not kept pace with the demands of clinical drug development. This work addresses that gap by developing a targeted proteomic approach that quantifies a Pseudomonas aeruginosa bacteriophage, LUZ19, through detection of a structural capsid protein, providing a host-independent, reproducible, and regulatorily defensible alternative to conventional plaque-based enumeration. The dataset includes discovery proteomics data used for structural protein characterization and signature peptide selection, as well as targeted quantitative mass spectrometry data generated during assay development and validation. This approach establishes a foundation for the pharmacokinetic and pharmacodynamic characterization of therapeutic phages and is directly scalable to the simultaneous quantitation of individual phage components within multi-phage cocktails. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-27 |
| AnnouncementXML | Submission_2026-05-27_18:03:34.214.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Thomas Nguyen |
| SpeciesList | scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; scientific name: Pseudomonas phage LUZ19; NCBI TaxID: 484896; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap XL; instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-24 18:00:21 | ID requested | |
| ⏵ 1 | 2026-05-27 18:03:34 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Bacteriophage, Phage, Pseudomonas aeruginosa, Quantitation, MRM, DatasetType:Proteomics |
Contact List
| Thomas D. Nguyen | |
|---|---|
| contact affiliation | University at Buffalo |
| contact email | tn34@buffalo.edu |
| lab head | |
| Thomas Nguyen | |
| contact affiliation | University at Buffalo |
| contact email | tn34@buffalo.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101576/ |
