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PXD077561
PXD077561 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global profiling of β-hydroxybutyrylation reveals OsACLA2 Kbhb enhances enzyme activity and rice blast resistance |
| Description | Lysine β-hydroxybutyrylation (Kbhb) is a metabolite-derived post-translational modification linking cellular metabolism to epigenetic and cellular regulation. Although studied in mammals, its landscape and functions in plants remain largely unknown. Here, we present the first proteome-wide analysis of Kbhb in rice leaves, identifying 656 modification sites on 482 proteins involved in diverse processes such as photosynthesis and central metabolism. We demonstrate that Kbhb on the conserved K262 residue of ATP-citrate lyase OsACLA2 enhances its enzymatic activity. This modification is essential for full rice blast resistance, as K262 site-specific mutants show compromised immunity. Furthermore, we identify the histone deacetylase OsHDA705 as an eraser enzyme that dynamically removes Kbhb from OsACLA2. Our findings reveal Kbhb as a crucial regulatory mechanism that integrates metabolic signaling with plant defense, establishing a novel pathway where the metabolite β-hydroxybutyrate fine-tunes metabolic enzyme activity to orchestrate effective immunity. This study provides a foundational resource and new insights for manipulating disease resistance in crops. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-24 |
| AnnouncementXML | Submission_2026-04-23_20:35:47.806.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuan Fang |
| SpeciesList | scientific name: Oryza sativa; NCBI TaxID: 4530; |
| ModificationList | acetylated residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-23 20:35:25 | ID requested | |
| ⏵ 1 | 2026-04-23 20:35:48 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: β-hydroxybutyrylation, ATP-citrate lyase, histone deacetylase, disease resistance |
Contact List
| Xiaoyang Chen | |
|---|---|
| contact affiliation | Anhui Agricultural University |
| contact email | 1149806052@qq.com |
| lab head | |
| Yuan Fang | |
| contact affiliation | Anhui Agricultural University |
| contact email | 1742813629@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
