PXD077559

PXD077559 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics profiling reveals immunometabolic adaptations underlying disease resilience in indigenous Lueyang Silky Fowl compared to White Leghorns
Description	Sustainable avian production demands genetic strategies beyond vaccines to enhance disease resilience, particularly in indigenous breeds like Lueyang Silky Fowl (LSF), which show superior resistance compared to commercial White Leghorn chickens (WLC) but lack molecular characterization. We hypothesized LSF breast muscle harbors integrated immune–metabolic adaptations absent in WLC, reflecting evolutionary divergences and physiological ecology. We performed RNA-seq, LC-MS/MS proteomics and untargeted metabolomics on sex- and age-matched LSF and WLC breast muscle (n=3/group). Differentially expressed genes (DEGs), proteins (DAPs), and metabolites (DAMs) were identified and integrated via pathway enrichment and network analyses. LSF muscle showed 2,577 DEGs (949 up, 1,628 down), 262 DAPs (48 up, 214 down), and 197 DAMs (52 up, 145 down). Concordant enrichments spanned mitochondrial oxidative phosphorylation; amino-acid metabolism (arginine/proline; alanine/aspartate/glutamate); MAPK and calcium signaling; and antioxidant pathways (notably glutathione). Integrated networks revealed 66 shared KEGG pathways, including convergent hubs in calcium/MAPK signaling, apelin, and focal adhesion, nominating candidate markers like NOS1, GOT1, PRKCA, MAP2K6, and ATP6V1E1. These bulk-tissue signatures likely integrate myocyte-intrinsic, stromal, and resident immune-cell programs; thus, offering testable signatures for avian immunometabolic resilience. We propose validation through single-cell/spatial transcriptomics, immunohistochemistry, targeted LC-MS and metabolomics, and pathogen challenge assays. This study advances ornithological understanding by revealing breed-specific physiological adaptations in poultry, positioning muscle as proxy for systemic resilience, and providing biomarkers for genomic selection to conserve and enhance genetic diversity in resilient avian lines under intensive farming pressure.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-26_21:04:24.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuang Liu
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-23 20:05:45	ID requested
⏵ 1	2026-05-26 21:04:24	announced

Dataset with its publication pending

submitter keyword: Lueyang Silky Fowl,Multi‑omics, immunometabolic resistance, avian disease resistance, biomarker discovery, White Leghorn

Xiaoying Zhang
contact affiliation	Shaanxi University of Technology
contact email	zhang@bio.uminho.pt
lab head
Shuang Liu
contact affiliation	Shaanxi University of Technology
contact email	13572623262@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077559
     1. Label: PRIDE project
     2. Name: Multi-Omics profiling reveals immunometabolic adaptations underlying disease resilience in indigenous Lueyang Silky Fowl compared to White Leghorns