PXD077541 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | qXL-MS of insulin resistance in mouse skeletal muscle |
| Description | We established a whole tissue qXL-MS workflow to investigate proteome-wide changes in protein:protein interactions in mouse skeletal muscle insulin resistance (IR). Mice were feed a standard chow diet or a high fat diet (HFD) for 12 weeks which induced skeletal muscle insulin resistance. The Extensor digitorum longus (EDL) skeletal muscles were isolated and subjected to qXL-MS analysis. We chose this small fast-twitch muscle because it weighs ~8-10 mg providing sufficient protein yield (~1 mg) and it is commonly used in ex vivo myograph preparations due to adequate oxygen diffusion in O2/CO2-carbogen gassed Krebs-Henseleit bicarbonate-based buffer (Barclay, 2005). For XL-MS analysis of IR in skeletal muscle, chow and HFD EDL muscles (n=8 each) were incubated with 10 mM tPhoX for 2h. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-29 |
| AnnouncementXML | Submission_2026-05-28_18:17:25.463.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Benjamin Parker |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-23 12:08:27 | ID requested | |
| ⏵ 1 | 2026-05-28 18:17:26 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: mouse skeletal muscle,insulin resistance |
Contact List
| Benjamin Parker |
|---|
| contact affiliation | The University of Melbourne |
| contact email | ben.parker@unimelb.edu.au |
| lab head | |
| Benjamin Parker |
|---|
| contact affiliation | The University of Melbourne |
| contact email | ben.parker@unimelb.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077541 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077541
- Label: PRIDE project
- Name: qXL-MS of insulin resistance in mouse skeletal muscle
