PXD077491

PXD077491 is an original dataset announced via ProteomeXchange.

Title	Atg7–Atg12 conjugation and autophagy are negatively regulated by the disordered region of Atg12
Description	: The macroautophagy/autophagy machinery has two ubiquitin-like (UBL) conjugation systems. The Atg8/MAP1LC3/GABARAP (yeast/human) and Atg12/ATG12 proteins are UBL substrates for Atg7/ATG7, a non-canonical E1 enzyme, that thioesterifies its substrates; however, autophagy requires a much greater amount of conjugated Atg8 (Atg8–PE) than Atg12 (Atg12–Atg5). Exactly how Atg7/ATG7 distinguishes between its two substrates to facilitate this differential biogenesis remains elusive. Here, analyses of recombinant complexes of yeast proteins reveal that the N termini of Atg8 and Atg12 are structural determinants for conjugation to Atg7, but play no role in conjugation to Atg3 or Atg10, non-canonical E2 enzymes. The disordered N terminus of Atg12 is a protector of the Atg12 C terminus and a negative regulator of Atg7–Atg12 conjugation and autophagy, whereas the N-terminal helical domain in Atg8 promotes autophagy and has a high avidity to Atg7. We show that balanced autophagy requires different specific N termini attached to the UBL domains.
HostingRepository	PRIDE
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-05_08:20:28.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hana Popelka
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-22 14:46:07	ID requested
⏵ 1	2026-05-05 08:20:29	announced

Dataset with its publication pending

submitter keyword: Atg3, Atg8, Atg10, ubiquitin-like

Hana Popelka
contact affiliation	Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
contact email	popelka@umich.edu
lab head
Hana Popelka
contact affiliation	University of Michigan
contact email	popelka@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077491

PRIDE project URI

• PRIDE
  1. PXD077491
     1. Label: PRIDE project
     2. Name: Atg7–Atg12 conjugation and autophagy are negatively regulated by the disordered region of Atg12