PXD077459

PXD077459 is an original dataset announced via ProteomeXchange.

Title	Competence-Driven Metabolism Triggers a Protein Aggregation and ROS Loop to Modulate Bacterial Adaptation to Chemical Cationic Biocides
Description	Antibiotic resistance is a growing concern, yet resistance and cross-resistance to widely used chemical biocides are poorly understood. Here, we show that cationic biocides induce V-type ATPase overactivation in Streptococcus gordonii, shifting metabolism to oxidative phosphorylation and increasing ROS. This triggers protein aggregation (PAs), which amplifies ROS in a lethal feedback loop. Synergistic inactivation of ClpX, a protease ATPase subunit, and PstB, a phosphate transporter component, disrupts the PAs-ROS loop by suppressing the competence regulator ComDE and rewiring metabolism to glycolysis. ClpX inactivation alleviates proteotoxic stress, promoting HtrA-mediated CSP degradation and ComDE downregulation, while PstB dysfunction impairs mannose PTS phosphorylation to further suppress ComDE. Reactivation of ComDE restores biocide susceptibility through the PAs-ROS loop. This mechanism is conserved in other ComDE-encoding streptococci, including Streptococcus pneumoniae, underscoring ComDE-mediated metabolic adaptation as a key regulator of bacterial susceptibility to membrane-targeted and ROS-inducing antimicrobials, and suggesting strategies to mitigate resistance to chemical biocides.
HostingRepository	iProX
AnnounceDate	2026-04-21
AnnouncementXML	Submission_2026-04-21_18:45:38.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Li
SpeciesList	scientific name: Streptococcus gordonii str. Challis substr. CH1; NCBI TaxID: 467705;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-21 18:45:17	ID requested
⏵ 1	2026-04-21 18:45:39	announced

Dataset with its publication pending

submitter keyword: Antimicrobial resistance, Cationic Biocides, Bacterial metabolism, Reactive Oxygen Species, Streptococcal Competence

Hao Li
contact affiliation	State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University
contact email	lihao106@shsmu.edu.cn
lab head
Hao Li
contact affiliation	State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University
contact email	lihao106@shsmu.edu.cn
dataset submitter

iProX dataset URI