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PXD077451
PXD077451 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Transcriptomic and Proteomic Signatures Underlying Nymphal Adaptation and Foam Production in the Forage Pest Mahanarva spectabilis. |
| Description | The spittlebug Mahanarva spectabilis (Distant, 1909) (Hemiptera: Cercopidae) is an important pest of forage grasses in South America, where its nymphs cause pasture damage by feeding on xylem sap and producing a characteristic foam that protects them against environmental stressors. To investigate the molecular basis of this adaptation, we integrated RNA-seq analysis of nymphs with LC-MS/MS proteomics of the Batelli gland, the primary source of foam secretion. De novo assembly of 100,666 unigenes revealed broad functional diversity, with strong representation of detoxification enzymes (CYP450s, GSTs, UGTs, carboxylesterases), transporters and ion pumps, cuticle proteins, and stress- and immunity-related genes. Nearly 16% of loci exhibited alternative splicing, particularly within detoxification, chemosensory, and osmoregulatory gene families, highlighting evidence of transcriptomic variability. Signal peptide and secreted protein predictions identified 168 high-confidence candidate secreted proteins, including detoxification enzymes, proteases, structural proteins, and immune-related factors, several of which are consistent with antimicrobial and surfactant-related functions for spittlebug foams. Proteomic profiling of the Batelli gland confirmed 500 proteins, enriched in chaperones, metabolic enzymes, detoxification pathways, and osmoregulatory components, with the most abundant proteins corresponding to Hsp70 chaperones, ATP synthases, cuticle proteins, and carbonic anhydrases. Together, these results provide an integrative transcriptomic and proteomic overview for M. spectabilis nymphs, highlighting genes and proteins associated with xylem feeding, foam production and responses potentially related to environmental stress tolerance. This comprehensive dataset not only advances the understanding of spittlebug biology but also identifies candidate molecular targets that may inform innovative strategies for controlling nymphal stages and mitigating spittlebug damage in forage systems. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-21 |
| AnnouncementXML | Submission_2026-04-21_14:59:45.738.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | EULALIO GUTEMBERG DIAS DOS SANTOS |
| SpeciesList | scientific name: Mahanarva spectabilis; NCBI TaxID: 1985197; |
| ModificationList | Lys->CamCys |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-21 14:53:04 | ID requested | |
| ⏵ 1 | 2026-04-21 14:59:46 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Forage grasses, Secretome, Plant-insect interactions, Detoxification enzymes, Transcriptomics, Proteomics, DatasetType:Proteomics |
Contact List
| Humberto Josue de Oliveira Ramos | |
|---|---|
| contact affiliation | Universidade Federal de Vicosa |
| contact email | eulaliobqi@gmail.com |
| lab head | |
| EULALIO GUTEMBERG DIAS DOS SANTOS | |
| contact affiliation | UFV |
| contact email | eulaliobqi@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101533/ |
