PXD077432

PXD077432 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling and LC-MS/MS based identification of the stress granule microenvironment induced by different viruses
Description	To dissect and compare the protein composition of stress granules formed during infections with two different RNA viruses as well as oxidative stress, we developed a recombinant mouse fibroblast cell line stably expressing a fusion protein of the stress granule marker G3BP1 with APEX2 and GFP. When stress granules are induced in these cells, the fusion protein accumulates in the cytoplasmic condensates and allows the biotinylation of the SG proteome based on the APEX2 peroxidase. The proximity labeled proteins can then be affinity purified and identified by quantitative LC-MS/MS. To directly compare different stressed and unstressed conditions, we combined the proximity labeling approach with stable isotope labeling of amino acids in cell culture (SILAC). In the first experiment (Exp 1, 2019 data), we compared the SGs induced by MHV infection with canonical SGs induced by sodium arsenite treatment or the unstressed cells. In a second and third experiment (Exp 2 and 3, 2023 and 2025 data), we expanded our investigations to SGs induced by SFV infection to directly compare the SG proteome between different viruses and at different time points during infection.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_00:01:19.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-21 05:05:46	ID requested
⏵ 1	2026-08-07 00:01:20	announced

10.1371/JOURNAL.PBIO.3003363;

submitter keyword: proximity labeling, mouse hepatitis virus, Semliki Forest virus,Stress granules

Volker Thiel
contact affiliation	Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland Department of Infectious Diseases and Pathobiology (DIP), Vetsuisse Faculty, University of Bern, Bern, Switzerland
contact email	volker.thiel@unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Departement for Biomedical Research, University of Berne
contact email	pmscf.dbmr@unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077432
     1. Label: PRIDE project
     2. Name: Proximity labeling and LC-MS/MS based identification of the stress granule microenvironment induced by different viruses