PXD077331 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bacteroides fragilis T6SS spike complex pulldowns |
| Description | We tested the hypothesis that the B. fragilis T6SS effector-toxin Bte3 forms a pre-secretion complex with its specific VgrG and the proposed accessory proteins encoded by orf2 and orf3. To test this, we generated functional pEV1/bte1_3(orf2/3) derivatives on the N1 t6-1 t6-2 background in which we HA-tagged Bte3 and alternatively Flag-tagged VgrG, Orf2, or Orf3. Reciprocal Flag-tag and HA-tag immunoprecipitations revealed protein-protein interactions between Bte3 and both Orf2 and Orf3. Anti-Flag immunoprecipitates of VgrG, Orf2, or Orf3 were subjected to mass spectrometry (MS), protein interactions supportive of a pre-secretion complex formed by Bte3, VgrG, Orf2 and Orf3 were detected in Orf2 and Orf3-tagged samples |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-05 |
| AnnouncementXML | Submission_2026-05-04_18:20:31.682.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD077331 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ezequiel Valguarnera |
| SpeciesList | scientific name: Bacteroides fragilis JCM 11017; NCBI TaxID: NEWT:1235816; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-17 14:00:58 | ID requested | |
| ⏵ 1 | 2026-05-04 18:20:32 | announced | |
Publication List
Keyword List
| submitter keyword: PAAR, T6SS,Bacteroides |
Contact List
| Juliane Bubeck Wardenburg |
|---|
| contact affiliation | Washington University in Saint Louis. School of Medicine, Department of Pediatrics, Critical Care Division. Saint Louis, MO, USA. |
| contact email | jbubeck@wustl.edu |
| lab head | |
| Ezequiel Valguarnera |
|---|
| contact affiliation | Washington University in Saint Louis |
| contact email | evalguarnera@wustl.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077331 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077331
- Label: PRIDE project
- Name: Bacteroides fragilis T6SS spike complex pulldowns
