PXD077315

PXD077315 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of native and decellularized rat submandibular gland scaffolds prepared using the S3T1 protocol
Description	Salivary gland hypofunction lacks effective regenerative treatment, and organ-specific decellularized extracellular matrix scaffolds represent a promising biomaterial platform for tissue engineering. In this study, rat submandibular glands were decellularized using a rapid S3T1 workflow consisting of freeze-thaw pretreatment, 1% SDS treatment for 3 h, and 0.25% trypsin-EDTA treatment for 1 h. Native submandibular glands and decellularized scaffolds were compared by data-independent acquisition LC-MS/MS to characterize protein composition and extracellular matrix preservation. The dataset was generated to identify retained matrisome components and to evaluate the proteomic features of the decellularized scaffold in comparison with native tissue. These data support the use of the decellularized submandibular gland scaffold as a bioactive platform for salivary gland tissue engineering.
HostingRepository	PRIDE
AnnounceDate	2026-04-21
AnnouncementXML	Submission_2026-04-20_19:50:28.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Gao
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	iodoacetamide derivatized residue
Instrument	quadrupole orbitrap astral instrument

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-17 09:17:11	ID requested
⏵ 1	2026-04-20 19:50:28	announced

Dataset with its publication pending

submitter keyword: extracellular matrix scaffold, proteomics, DIA, rat,Submandibular gland, salivary gland tissue engineering, decellularization, LC-MS/MS

Jie Gao
contact affiliation	Shanghai Jiao Tong University
contact email	1210905087@qq.com
lab head
Jie Gao
contact affiliation	Shanghai Jiao Tong University
contact email	1210905087@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD077315

PRIDE project URI

• PRIDE
  1. PXD077315
     1. Label: PRIDE project
     2. Name: Proteomic characterization of native and decellularized rat submandibular gland scaffolds prepared using the S3T1 protocol