PXD077305

PXD077305 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum PfMyoF with a Rab-like tail domain associates with perinuclear membranes and trafficking proteins
Description	Members of the myosin superfamily are found in all eukaryotes, including Plasmodium falciparum, the parasite that causes the majority of severe malaria. The P. falciparum genome encodes six myosins, but apart from PfMyoA and B, these remain largely uncharacterised. Here, we characterise PfMyoF, a class XXII myosin, using structural prediction, biochemical assays, and imaging. We show that PfMyoF is a plus-end-directed, processive motor with a long neck region with capacity to bind up to six copies of the calmodulin homologue PfCaM. The PfMyoF tail contains predicted WD40 and Rab-like domains that have not been identified in any other eukarytotic myosin class. Pull-down experiments identify PfMyoF interactions with trafficking proteins, including the vesicle marker PfRab18. Expansion and immunoelectron microscopy reveal that PfMyoF localises to a perinuclear membrane compartment and transient knockdown in using the glmS ribozyme system impairs growth, potentially indicating an important function during asexual replication. Our findings define PfMyoF as the first myosin with a Rab-like domain and highlight its potential role in membrane trafficking pathways during the pathogenic blood stages of parasite development.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-27_02:35:25.465.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robin Antrobus
SpeciesList	scientific name: Plasmodium (Paraplasmodium); NCBI TaxID: NEWT:418106;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-17 05:26:37	ID requested
⏵ 1	2026-04-27 02:35:26	announced

Dataset with its publication pending

submitter keyword: malaria,Myosin, IP-MS, trafficking

Folma Buss
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge; Cambridge, UK
contact email	fb207@cam.ac.uk
lab head
Robin Antrobus
contact affiliation	CIMR medicine
contact email	pra29@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077305
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum PfMyoF with a Rab-like tail domain associates with perinuclear membranes and trafficking proteins