PXD077261

PXD077261 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Bclaf1 Drives Heart Failure by Recruiting Srsf2 to Enhance Hand2 Pre-mRNA Splicing and Pathological Hypertrophy
Description	Heart failure with reduced ejection fraction (HFrEF) remains a major therapeutic challenge. Bcl2-associated transcription factor 1 (Bclaf1) is implicated in RNA splicing and cardiac disease, but its role in HFrEF pathogenesis is unknown. Here, we demonstrate that Bclaf1 expression is elevated in human HFrEF myocardium and in murine pressure-overload models. Cardiac-specific Bclaf1 overexpression drives pathological hypertrophy and systolic dysfunction, whereas its genetic knockout or AAV9-mediated knockdown attenuates these phenotypes. Mechanistically, Bclaf1 interacts with the splicing factor Srsf2 to bind and enhance the splicing efficiency of Hand2 pre-mRNA, leading to increased mature Hand2 levels and maladaptive remodeling. Inhibition of either Bclaf1 or Hand2 rescues cardiac function and structure in experimental HFrEF. Our work defines a novel Bclaf1/Srsf2/Hand2 splicing axis as a critical driver of HFrEF and reveals a promising therapeutic target for heart failure.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_06:38:07.463.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yang zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-16 12:55:33	ID requested
⏵ 1	2026-05-27 06:38:08	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Bclaf1
Hand2
HFrEF
RNA splicing
therapeutic target

submitter keyword: Bclaf1

Hand2

HFrEF

RNA splicing

therapeutic target

Contact List

yang zhang
contact affiliation	harbin medical university
contact email	46506962@qq.com
lab head
yang zhang
contact affiliation	Harbin Medica university
contact email	46506962@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077261
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077261

PRIDE project URI

Repository Record List

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