⮝ Full datasets listing
PXD077233
PXD077233 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Arid3b suppresses CD8+ T cell infiltration and function in Microsatellite-stable colorectal cancer via Runx3 |
| Description | Microsatellite stable/proficient mismatch repair (MSS/pMMR) colorectal cancer (CRC) is characterized by a cold tumor microenvironment, marked by sparse CD8⁺ T cell infiltration and resistance to immune checkpoint inhibitors (ICIs). To uncover regulators that limit CD8⁺ T cell infiltration in MSS CRC, we conducted an in vivo CRISPR/Cas9 screen targeting genes in CD8⁺ T cells using a CMT93 cell-derived tumor model. This screen identified Arid3b as a key negative regulator of CD8⁺ T cell tumor infiltration and antitumor activity. Genetic ablation of Arid3b in CD8⁺ T cells significantly enhanced their intratumoral accumulation and mediated robust tumor control. Mechanistically, Arid3b deficiency upregulated Runx3, promoting a tissue-resident memory phenotype and effector function. Notably, the benefits conferred by Arid3b knockout were abrogated by Runx3 deletion, indicating a Runx3-dependent mechanism. In summary, targeting ARID3B could offer a promising strategy to reshape the tumor microenvironment and sensitize MSS CRC to immunotherapy. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-16 |
| AnnouncementXML | Submission_2026-06-01_19:33:43.541.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shuo Wang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-16 02:29:06 | ID requested | |
| 1 | 2026-04-16 02:32:39 | announced | |
| ⏵ 2 | 2026-06-01 19:33:44 | announced | 2026-06-02: Add info |
Publication List
| Wang S, Hou S, Luo C, Zhang H, Jin Y, Zhang R, Zhao Y, Xiong X, Guo R, Wang C, Bao Y, Wen L, Pan D, Ye Y, Zeng Z, Gao Z, T cell infiltration and function in microsatellite-stable colorectal cancer via Runx3. Nat Commun, ():(2026) [pubmed] |
Keyword List
| submitter keyword: ARID3B, IP/MS, CD8+ T cell, MSS, colorectal cancer |
Contact List
| Zhidong Gao | |
|---|---|
| contact affiliation | Peking University People’s Hospital |
| contact email | gaozhidong@pkuph.edu.cn |
| lab head | |
| Shuo Wang | |
| contact affiliation | Peking University People’s Hospital |
| contact email | dr_shuowang@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
