PXD077230

PXD077230 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of differentially expressed proteins in TMBIM5-overexpressing cells under PA stimulation compared to control PA-treated AML12 cells
Description	This project aims to identify differentially expressed proteins between the Ctrl+PA group and the OE-TMBIM5+PA group using proteomic analysis. Cells were lysed with RIPA buffer containing protease inhibitors, followed by ultrasonication and centrifugation to collect the supernatant. Proteins were dissolved in 8 M urea, reduced with DTT, alkylated with 50 mM iodoacetamide, and digested overnight with trypsin. The resulting peptides were desalted using C18 tips and analyzed by liquid chromatography coupled with tandem mass spectrometry (Q Exactive HF-X). This approach allows for the identification of protein expression changes associated with TMBIM5 overexpression under PA treatment.
HostingRepository	iProX
AnnounceDate	2026-04-17
AnnouncementXML	Submission_2026-04-19_17:22:28.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Shen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-16 02:06:02	ID requested
⏵ 1	2026-04-19 17:22:29	announced

Dataset with its publication pending

submitter keyword: TMBIM5, proteomics, PA stimulation, mass spectrometry

Mianyang Li
contact affiliation	Department of Laboratory Medicine, The First Medical Center of Chinese PLA General Hospital
contact email	Limianyang7772@163.com
lab head
Qi Shen
contact affiliation	Department of Laboratory Medicine, The First Medical Center of Chinese PLA General Hospital
contact email	17835157589@163.comom
dataset submitter

iProX dataset URI