PXD077197

PXD077197 is an original dataset announced via ProteomeXchange.

Title	Matricytosis is a phenotype specific ECM internalization program dysregulated across macrophages and fibroblasts in idiopathic pulmonary fibrosis
Description	Idiopathic pulmonary fibrosis (IPF) is characterized by persistent extracellular matrix (ECM) accumulation, yet the mechanisms governing ECM clearance remain poorly understood. In this study we investigate matricytosis, a program of ECM uptake and lysosomal degradation, and its status in disease. We show that matricytosis is highly phenotype-dependent in human macrophages and fibroblasts: disease-associated macrophages have impaired ECM internalization, while myofibroblasts demonstrate increased uptake. Inhibition studies, single-cell RNA sequencing pathway analysis and spatial transcriptomics reveal distinct uptake mechanisms for each cell type, and highlight macrophages as dominant uptake-proficient cells in both healthy and fibrotic lung tissue, while fibroblast matricytosis is linked to invasive programs prominent in IPF. Using human decellularized precision‑cut lung slices, we demonstrate that fibrotic matrix affects matricytosis and modulates cell behavior. In this work, we show that matricytosis depends on effector cell type, its phenotype and matrix environment, is dysregulated in IPF and thus, presents a potential therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_02:18:03.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Kallabis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	quadrupole orbitrap astral instrument

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-15 11:46:55	ID requested
⏵ 1	2026-08-18 02:18:04	announced

Dataset with its publication pending

submitter keyword: Idiopathic pulmonary fibrosis, matricytosis, extracellular matrix

Wioletta Skronska-Wasek
contact affiliation	Boehringer Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riß, Germany
contact email	wioletta.skronska-wasek@boehringer-ingelheim.com
lab head
Sebastian Kallabis
contact affiliation	Core Facility Translational Proteomics Medical Faculty University Bonn Venusberg - Campus 1 53127 Bonn Germany
contact email	kallabis@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD077197

PRIDE project URI

• PRIDE
  1. PXD077197
     1. Label: PRIDE project
     2. Name: Matricytosis is a phenotype specific ECM internalization program dysregulated across macrophages and fibroblasts in idiopathic pulmonary fibrosis