⮝ Full datasets listing
PXD077145
PXD077145 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Extracellular Vesicles from Chemoresistant Breast Cancer Cells Reprogram Macrophages Toward an M2-Like Phenotype in an ABCC1-dependednt manner |
| Description | Triple-negative breast cancer is characterized by high rates of chemoresistance and poor clinical outcomes. Although ATP binding cassette (ABC) transporters are well-established mediators of drug efflux, their role in tumor immune communication remains poorly defined. Here, we investigated whether acquisition of doxorubicin resistance alters extracellular vesicle (EV) biogenesis and cargo content. The doxorubicin resistant MDA MB 231 cell line demonstrated significantly higher rates of EV production, without changes in particle size distribution, compared the parental cell line. At the cellular level, we found an increase in the number of multivesicular bodies in the resistant cell line. Proteomic and western blot analysis of isolated EVs derived from the doxorubicin resistant MDA MB 231 cell line compared to the parental, MDA MB 231, cell line revealed an enrichment of ABCC1, accompanied by enrichment of vesicle associated pathways. Cellular ABCC1 localized to CD63 positive compartments and was validated as enriched in resistant cell line derived EVs. Functionally, EVs from resistant cells promoted M2 like polarization of primary human macrophages, characterized by decreased CD86 positive and increased CD206 positive populations. Knocking down ABCC1 in resistant cells significantly attenuated this effect. Together, these findings suggest EV associated ABCC1 might be a contributor to macrophage polarization and immune modulation in breast cancer. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-14 |
| AnnouncementXML | Submission_2026-04-14_10:48:04.255.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jason Rogalski |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-14 10:28:49 | ID requested | |
| ⏵ 1 | 2026-04-14 10:48:04 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Extracellular Vesicles, Breast Cancer, DatasetType:Proteomics |
Contact List
| Karla C. Williams | |
|---|---|
| contact affiliation | The University of British Columbia |
| contact email | karla.williams@ubc.ca |
| lab head | |
| Jason Rogalski | |
| contact affiliation | UBC |
| contact email | rogy@mail.ubc.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101462/ |
