⮝ Full datasets listing
PXD077129
PXD077129 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The secretory peptidase inhibitor R3HDML fine-tunes p38 MAPK phosphorylation and mesangial phenotypes during renal aging and disease |
| Description | Serine proteases and their inhibitors are the most abundant class of proteolytic enzymes, involved in numerous biological processes such as cellular crosstalk. However, their role in renal diseases remains controversial. In this study, we identify R3HDML as highly podocyte specific expressed and secreted putative serine peptidase inhibitor. CRISPR/Cas9-mediated generation of a novel floxed-R3hdml allele, demonstrated a regulatory function in renal aging and streptozotocin induced diabetic nephropathy. R3hdml knockout mice developed a hump-like thickening of the glomerular basement membrane, as well as mesangial expansion. Despite these morphological alterations, renal function was not impaired by loss of R3hdml in aging, diabetic nephropathy, and ischemia-reperfusion induced tubular injury and regeneration. Employing interactome analyses led to the identification of p38 MAPK signaling-related kinases, and N-terminal signaling peptide containing proteins, as R3HDML interactors. Consequently, activating Thr180/Tyr182 phosphorylation of p38 MAPK was suppressed by R3HDML in podocytes. In summary, we demonstrate a highly selective expression of the putative serine peptidase inhibitor R3HDML in podocytes and a protective regulatory role in kidney disease and aging. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-20 |
| AnnouncementXML | Submission_2026-05-20_23:39:21.010.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stefan Tholen |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-14 05:57:02 | ID requested | |
| ⏵ 1 | 2026-05-20 23:39:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: R3h domain containing-like, R3HDML, Podocyte, MAPK, AKI, Diabetes, CKD, DatasetType:Proteomics |
Contact List
| Dr. Wibke Bechtel-Walz | |
|---|---|
| contact affiliation | University Medical Center Freiburg |
| contact email | wibke.bechtel@uniklinik-freiburg.de |
| lab head | |
| Stefan Tholen | |
| contact affiliation | Uniklinik Freiburg |
| contact email | stefan.tholen@uniklinik-freiburg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101459/ |
