PXD077056

PXD077056 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of proteins interacting with HBoV1 NS1, NS2, and NS1-70
Description	HEK293T cells in 10 cm dishes were transfected with 10 μg of HA-tagged viral protein plasmid, and total protein was collected 48 h after transfection. Cells were lysed in lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EGTA, 2 mM DTT, 100 μM PMSF, 1 μg/mL protease inhibitor cocktail) on ice for 30 min. Primary antibodies were mixed with supernatants of cell lysates (2 μg primary antibody per 1 mg protein sample) overnight at 4˚C and then incubated with Dynabeads™ Protein G/A for 4 h at 4˚C. Beads were washed 3 times with wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton X-100, 1 mM EGTA), 3 times with high-salt wash buffer (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 0.1% Triton X-100, 1 mM EGTA), and 3 times with 1× PBS to eliminate non-specific protein binding. After retaining 1/10 of the beads for western blot validation of IP efficiency, the remaining beads were sent to SpecAlly Life Technology Co., Ltd. for label-free quantitative mass spectrometry (MS) analysis.
HostingRepository	iProX
AnnounceDate	2026-04-11
AnnouncementXML	Submission_2026-04-12_20:12:10.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuchu Tian
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-12 20:11:32	ID requested
⏵ 1	2026-04-12 20:12:10	announced

Dataset with its publication pending

submitter keyword: HBoV1 non-structural protein

Yuning Sun
contact affiliation	Ningxia Medical University
contact email	sunyuning1994@nxmu.edu.cn
lab head
Chuchu Tian
contact affiliation	Ningxia Medical University
contact email	tiancc07@163.com
dataset submitter

iProX dataset URI