PXD076866 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Adaptation of immunoaffinity protocol for the isolation of the cell line immunopeptides - proteomics part |
| Description | BACKGROUND: The peptide repertoire presented by Human Leukocyte Antigens (HLA) provides a snapshot of intracellular proteins, which is monitored by the immune system. Although mass spectrometry is the gold standard for identification of these peptides, the upstream steps of the immunopeptidomics workflow, including immunoprecipitation and peptides purification, vary and critically impact the peptide yield.
AIM: To optimize conditions for the isolation of a larger number of HLA I ligands.
METHODS: Immunopeptidomes from cell lines were isolated using immunoaffinity followed by liquid chromatography-mass spectrometry analysis. Cell lines were lysed with various detergents (CHAPS, NP-40, SOD, Triton X-100) and eluted with TFA, urea, or thiocarbamate solutions.
RESULTS: We optimized a protocol for immunoprecipitating peptide-HLA complexes and found that the number of identified peptides scales with the number of input cells, while being unaffected by the choice of lysis buffer.
CONCLUSION: The optimized protocol allows the isolation of HLA I ligand peptides from suspension cell lines. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-15 |
| AnnouncementXML | Submission_2026-04-15_09:51:50.108.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD076866 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Georgij Arapidi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-10 04:47:37 | ID requested | |
| ⏵ 1 | 2026-04-15 09:51:50 | announced | |
Publication List
Keyword List
Contact List
| Georgij Arapidi |
|---|
| contact affiliation | Head of System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| lab head | |
| Georgij Arapidi |
|---|
| contact affiliation | System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076866
- Label: PRIDE project
- Name: Adaptation of immunoaffinity protocol for the isolation of the cell line immunopeptides - proteomics part
