PXD076847

PXD076847 is an original dataset announced via ProteomeXchange.

Title	Lysine Acetylation of Translation Initiation Factor IF-2 Mediated by Mycobacterial Acetyltransferase Eis
Description	In our study, we employed the non-pathogenic Mycobacterium smegmatis as a model organism and applied a multi-omics approach to elucidate the substrate specificity and regulatory function of the mycobacterial acetyltransferase Eis. Through the integration of TMT-based acetyl-proteomics, dimethyl labeling-based acetyl-proteomics, and protein interactome analyses, we identified lysine 509 of the translation initiation factor IF-2 (infB), which is involved in ribosomal function, as a novel substrate of Eis. Acetylation at K509 was found to inhibit the GTPase activity of infB, leading to a decrease in the biosynthesis of nascent proteins. Furthermore, we demonstrated that Eis-mediated acetylation of infB reduced the tolerance of bioengineered M. smegmatis strain to the ribosome-targeting antibiotic clarithromycin and increased the production of inflammatory cytokines infected in the macrophage THP-1 cell line and mouse kidney. Our findings elucidate the Eis-infB regulatory axis, highlighting the critical roles of infB K509 acetylation in modulating mycobacterial homeostasis, ribosomal function, and host immune responses.
HostingRepository	iProX
AnnounceDate	2026-04-10
AnnouncementXML	Submission_2026-04-10_00:52:43.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junyu Xu
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 51; NCBI TaxID: 1445611;
ModificationList	acetylated residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-10 00:52:27	ID requested
⏵ 1	2026-04-10 00:52:44	announced

Dataset with its publication pending

submitter keyword: Mycobacteria, Lysine acetylation, Acetyltransferase Eis, Translation initiation factor IF-2 K509

Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
lab head
Junyu Xu
contact affiliation	Chinese Academy of Sciences Shanghai Institute of Materia Medica
contact email	jyxu@simm.ac.cn
dataset submitter

iProX dataset URI