PXD076818

PXD076818 is an original dataset announced via ProteomeXchange.

Title	Cryo-electron tomography and proteomics identify a virus structure poised for genome uncoating
Description	Viral infections invariably require the dismantling of capsids enclosing viral genomes. Yet, discrete intermediates and host factors supporting nucleic acid uncoating in susceptible cells are largely elusive. Using cryo-electron tomography (cryo-ET) and superresolution microscopy we show that human cells lacking the ubiquitin E3-ligase Mindbomb-1 (Mib1) deadlock adenovirus (AdV) particles at ~150nm radial distance from the nuclear pore complex (NPC). Cryo-ET revealed that the NPC-docked intermediate particles exhibit a central cavity in hexon trimers, the major capsid protein. Quantitative proteomics of isolated nuclei indicated that the intermediate particles bear reduced amounts of fiber and penton-base essential to attach the incoming virus particles to plasma membrane receptors, as well as reduced inner proteins VI and X used for endosomal membrane disruption and charge neutralization of viral DNA, respectively. The intermediate particles colocalized with the host nuclear export factor CRM1/exportin-1, independent of RanGTP hydrolysis required for CRM1 cargo export. They detached from the NPC upon CRM1 inhibition by the small compound leptomycin B, and redocked after inhibitor wash-out, indicating a tunable, direct function of CRM1 in NPC tethering of the intermediate particle poised for DNA uncoating. The results have implications on anti-viral strategies, gene therapy and synthetic biology.
HostingRepository	PRIDE
AnnounceDate	2026-04-14
AnnouncementXML	Submission_2026-04-14_11:50:30.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alfonso Gomez Gonzalez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5); NCBI TaxID: NEWT:28285;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-09 13:09:37	ID requested
⏵ 1	2026-04-14 11:50:31	announced

Dataset with its publication pending

submitter keyword: None

Urs Greber
contact affiliation	Department of Molecular Life Sciences University of Zurich Winterthurerstrasse 190 CH-8057 Zurich Switzerland
contact email	urs.greber@mls.uzh.ch
lab head
Alfonso Gomez Gonzalez
contact affiliation	Whitehead Institute, MIT
contact email	alfonsogomez3@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076818

PRIDE project URI

• PRIDE
  1. PXD076818
     1. Label: PRIDE project
     2. Name: Cryo-electron tomography and proteomics identify a virus structure poised for genome uncoating