PXD076805

PXD076805 is an original dataset announced via ProteomeXchange.

Title	Tubulin autoregulation tunes microtubule dynamics to support multicellular architecture and viability
Description	Abstract Alpha- and beta-tubulin heterodimers dynamically assemble into microtubules, key cytoskeletal elements involved in intracellular trafficking, cell adhesion, and division. The availability of free tubulins regulates the synthesis of new subunits. In response to excessive soluble αβ-tubulins, tetratricopeptide protein 5 (TTC5) selectively recognizes nascent tubulins at the ribosome, recruiting downstream effectors that degrade their encoding messenger RNAs, in a process known as tubulin autoregulation. Despite its well-characterized molecular framework, the biological relevance of this regulatory pathway remains unknown. Here, using human 3D cellular models, advanced optics, and genetic perturbation of tubulin biosynthesis, we reveal that loss of TTC5-dependent tubulin autoregulation elevates soluble tubulin levels, increasing microtubule stability and disrupting cytoskeletal organization. These defects impair the localization of adhesion molecules at cell-cell junctions and extracellular matrix interfaces, compromising tissue architecture and reducing overall cell viability. Our findings establish tubulin autoregulation as a critical mechanism that tunes microtubule dynamics to sustain cellular integrity and tissue homeostasis. Note: quantitative mass spectrometry was used to determine the relative abundance of alpha- and beta-tubulin isotypes, as well as, adhesion proteins in TTC5 KO versus parental spheroids.
HostingRepository	PRIDE
AnnounceDate	2026-06-17
AnnouncementXML	Submission_2026-06-17_05:06:46.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Coelho Almeida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-09 13:02:08	ID requested
⏵ 1	2026-06-17 05:06:47	announced

Dataset with its publication pending

submitter keyword: LC-MS, HeLa, Cytoskeleton, Spheroids,Human cells, Tubulin

Ivana Gasic
contact affiliation	Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland
contact email	ivana.gasic@unige.ch
lab head
Ana Coelho Almeida
contact affiliation	Department of Molecular and Cellular Biology, University of Geneva, Geneva, Switzerland
contact email	ana.almeida@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD076805

PRIDE project URI

• PRIDE
  1. PXD076805
     1. Label: PRIDE project
     2. Name: Tubulin autoregulation tunes microtubule dynamics to support multicellular architecture and viability