PXD076788 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification and phosphorylation site mapping of a sperm specific PKA C subunit isoform from Mytilus galloprovincialis |
| Description | This project investigates a sperm-specific isoform of the catalytic (C) subunit of cAMP-dependent protein kinase (PKA) in the marine mussel Mytilus galloprovincialis, an externally fertilizing invertebrate widely used as a model for gamete activation studies. A C-subunit isoform purified from male gonadal tissue was identified by LC–MS/MS as the protein corresponding to UniProt entry A0A8B6GIP6. Sequence analysis revealed high structural conservation with mammalian Cα2 within the catalytic core, but also demonstrated the presence of a markedly extended, acidic N-terminal region unique to mussel sperm. Comprehensive phosphoproteomic profiling identified phosphorylation at Ser5 and Thr30 within the N-terminal extension, as well as a likely regulatory phosphosite at Thr200 located in the activation loop of the kinase catalytic core. Phosphorylation events detected in unenriched samples were independently validated through TiO₂ phosphopeptide enrichment followed by LC–MS/MS. These findings reveal structural and regulatory motifs not present in mammalian sperm PKA isoforms and suggest the existence of molluscan-specific mechanisms controlling sperm activation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-07_04:21:19.664.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Susana Bravo |
| SpeciesList | scientific name: Crenomytilus; NCBI TaxID: NEWT:151217; |
| ModificationList | phosphorylated residue |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-09 12:56:08 | ID requested | |
| ⏵ 1 | 2026-09-07 04:21:20 | announced | |
Publication List
| 10.1002/mrd.70139; |
| Ibarguren I, Flament-Simon SC, Losada AP, Bravo SB, Villamar, í, n JA, Mussel Spermatozoa Express a Specific Isoform of the Catalytic Subunit of cAMP-Dependent Protein Kinase. Mol Reprod Dev, 93(8):e70139(2026) [pubmed] |
Keyword List
| submitter keyword: cAMP-dependent protein kinase |
| PKA |
| catalytic subunit |
| LC–MS/MS |
| phosphoproteomics |
| phosphorylation sites |
| TiO₂ enrichment |
| Mytilus galloprovincialis |
| sperm |
Contact List
| Susana B Bravo |
|---|
| contact affiliation | Instituto de >Investigaciones Sanitarias de Santiago de Compostela |
| contact email | sbbravo@gmail.com |
| lab head | |
| Susana Bravo |
|---|
| contact affiliation | FIDIS |
| contact email | sbbravo@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076788 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076788
- Label: PRIDE project
- Name: Identification and phosphorylation site mapping of a sperm specific PKA C subunit isoform from Mytilus galloprovincialis
