PXD076776

PXD076776 is an original dataset announced via ProteomeXchange.

Title	Multiomics dissection of the BRAF V600E mutation-induced cellular state transition in the lung epithelial cells
Description	Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer, and 4% of NSCLC patients are reported to harbor mutations in BRAF, which encodes a serine/threonine kinase capable of activating the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway. However, the role and effect of BRAF mutation in NSCLC or lung lineage cells are not fully understood. To mimic the naturally occurring BRAF V600E mutation in lung cancer, a BRAF V600E knock-in BEAS-2B cell model was established using CRISPR/Cas9. The cancer cell biology of the BRAF V600E knock-in BEAS-2B cell was characterized by CCK8 assay, colony formation assay and Transwell assay. The proteomics and untargeted metabolomics were carried out to evaluate the underlying changes at the proteome and metabolome levels, respectively. The levels of specific proteins were examined by Western blotting. Although the BRAF V600E mutation alone was not sufficient to drive lung carcinogenesis, it induced remarkable changes in cellular migration capacity and tumorigenic potential. Proteomics analysis revealed significant changes in the proteins involved in the biological processes including epithelial-mesenchymal transition (EMT), extracellular matrix (ECM)-receptor interaction, cell adhesion, focal adhesion, and cell metabolism upon BRAF V600E mutation. Untargeted metabolomics experiments revealed that amino acid metabolism, especially the glycine, serine and threonine metabolism, was significantly modulated in BRAF V600E knock-in cells. In summary, our present study provides a new isogenic cell model that allows us to systematically dissect the underlying molecular events involved in the transition of cellular states upon the introduction of the BRAF V600E mutation.
HostingRepository	iProX
AnnounceDate	2026-04-08
AnnouncementXML	Submission_2026-04-08_23:48:44.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingyun Dai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-08 23:47:21	ID requested
⏵ 1	2026-04-08 23:48:45	announced

Dataset with its publication pending

submitter keyword: BRAF, V600E, Lung, NSCLC, amino acid metabolism

Lingyun Dai
contact affiliation	the First Affiliated Hospital of Southern University of Science and Technology
contact email	lingyun.dai@outlook.com
lab head
Lingyun Dai
contact affiliation	the First Affiliated Hospital of Southern University of Science and Technology
contact email	lingyun.dai@outlook.com
dataset submitter

iProX dataset URI