PXD076696

PXD076696 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry-Based Urinary Proteomic Analysis in Feline Chronic Kidney Disease
Description	Chronic kidney disease (CKD) is highly prevalent in domestic cats (Felis catus), and the specie is a potential model for human CKD. Mass spectrometry-based urine proteomic analysis is a promising tool for CKD diagnosis and management in human beings; yet, minimal information is available in cats. This study aims to explore alterations in the urinary proteome in feline CKD and assess its diagnostic value. Urine from 38 cats (14 with surgically induced CKD, 24 healthy) was used as training data. High-performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS) was used for urine proteomic analysis, and proteins’ quantities were calibrated to a spike-in protein serving as internal standard. Principal component analysis (PCA), logistic regression analysis, and functional enrichment analysis revealed and characterized changes in the urinary proteome in cats with CKD, compared to healthy cats. A random forest model was trained and applied to a second set of cats with naturally occurring CKD and a corresponding control group (total n= 40; 23 cats with CKD, 17 healthy controls). Under receiver-operating-characteristic (ROC) curve analysis, the model gave an area under curve (AUC) of 0.985 (95% CI, 0.959-1.000), outperforming serum symmetric dimethylarginine (SDMA), urine protein-to-creatinine ratio (UPC), and some commonly studied urinary biomarkers (i.e., transferrin, albumin, cystatin c). Retinol binding protein 4 (RBP4), the top contributor of the random forest model, achieved a comparable AUC of 0.977 (95% 0.941-1.000). These data provide proof of concept for mass spectrometry-based urinary proteomic analysis application in feline CKD.
HostingRepository	PRIDE
AnnounceDate	2026-08-22
AnnouncementXML	Submission_2026-08-22_08:53:44.333.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jane Huang
SpeciesList	scientific name: Felis catus (Cat) (Felis silvestris catus); NCBI TaxID: NEWT:9685;
ModificationList	No PTMs are included in the dataset
Instrument	impact

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-07 09:02:32	ID requested
⏵ 1	2026-08-22 08:53:44	announced

10.14814/PHY2.71072;

submitter keyword: CKD,Cat, Remnant Kidney Model, Chronic Kidney Disease, Urine

Bianca N Lourenço
contact affiliation	Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
contact email	lourenco@uga.edu
lab head
Jane Huang
contact affiliation	University of Georgia
contact email	hjh69772@uga.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD076696

PRIDE project URI

• PRIDE
  1. PXD076696
     1. Label: PRIDE project
     2. Name: Mass Spectrometry-Based Urinary Proteomic Analysis in Feline Chronic Kidney Disease