PXD076681

PXD076681 is an original dataset announced via ProteomeXchange.

Title	Tvp complexes support formation of the VgrG-PAAR spike during Type VI secretion system assembly
Description	Type VI secretion systems (T6SSs) are nanomachineries used by bacteria to inject toxic effectors into neighbouring cells during interbacterial competition or infection. The existence of multiple paralogues of the structural proteins VgrG and PAAR and of specific accessory proteins allows the same T6SS to deliver a wide range of effectors. We describe a new set of accessory proteins required for assembly of T6SSs containing the VgrG1-Paar1 spike and delivery of a novel VgrG1-associated membrane-targeting effector in Serratia marcescens. TvpAB, TvpB, TvpC and Paar1 form a pre-complex essential for T6SS assembly, with VgrG1 subsequently replacing TvpC. Phylogenetic analysis and structural modelling reveal that Tvp proteins are widely conserved but Tvp-containing complexes vary in organisation and complexity. We define three classes of Tvp complex, all sharing a DUF2169-containing TvpA protein which stabilises a DUF4150/PAAR-containing protein. Class I, which includes only TvpA, functions without a pre-complex, whereas Classes II and III involve additional Tvp components and two-step assembly. Our findings highlight how modular effector recruitment strategies underlie the versatility of the T6SS and suggest how alternative Tvp systems are tailored to promote assembly of secretion-competent, effector-loaded VgrG-PAAR spikes.
HostingRepository	PRIDE
AnnounceDate	2026-05-30
AnnouncementXML	Submission_2026-05-30_13:59:36.102.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Coulthurst
SpeciesList	scientific name: Serratia marcescens; NCBI TaxID: NEWT:615;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-07 07:12:04	ID requested
⏵ 1	2026-05-30 13:59:36	announced

10.1038/S44318-026-00820-1;

submitter keyword: Serratia marcescens, VgrG proteins, Tvp complexes, Type VI secretion system

Sarah Coulthurst
contact affiliation	School of Life Sciences, University of Dundee
contact email	s.j.coulthurst@dundee.ac.uk
lab head
Sarah Coulthurst
contact affiliation	University of Dundee
contact email	s.j.coulthurst@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD076681

PRIDE project URI

• PRIDE
  1. PXD076681
     1. Label: PRIDE project
     2. Name: Tvp complexes support formation of the VgrG-PAAR spike during Type VI secretion system assembly