PXD076622

PXD076622 is an original dataset announced via ProteomeXchange.

Title	Ubiquitination regulates granulostasis and DRiP accumulation in SGs under heat stress via the E3 ligase MKRN2
Description	Stress granules (SGs) are transient cytoplasmic biomolecular condensates that play a role in the cellular response to proteotoxic stress. It has been previously shown that ubiquitination regulates SG dynamics; however, the specific mechanisms by which ubiquitin affects SGs are not fully understood. Here, using proximity proteomics, we discover that the engagement of several E3 ubiquitin ligases in SGs is dependent on the activity of the E1 ubiquitin ligase UBA1. A detailed study of the E3 ubiquitin-protein ligase Makorin 2 (MKRN2) demonstrated that it is localized to SGs in a manner dependent on UBA1 activity. MKRN2 promotes both the proper formation of SGs and their disassembly following stress recovery, by preventing the accumulation of defective ribosomal products (DRiPs) within SGs. Therefore, MKRN2 is a novel regulator of SGs that mediates the maintenance of granulostasis, suggesting that the localization of a subset of E3 ligases into SGs is linked to UBA1 activity.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_01:48:18.567.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuel Amzallag
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-05 20:44:18	ID requested
⏵ 1	2026-09-09 01:48:19	announced

10.1038/s44319-026-00832-2;

Amzallag E, Danino YM, Secco V, Carra S, Hornstein E, The E3 ligase MKRN2 prevents DRiP accumulation in stress granules and maintains granulostasis. EMBO Rep, 27(15):4238-4251(2026) [pubmed]

submitter keyword: stress granules ubiquitin

Eran Hornstein
contact affiliation	Department of Molecular Genetics, Weizmann Institute of Science, Israel.
contact email	eran.hornstein@weizmann.ac.il
lab head
Emmanuel Amzallag
contact affiliation	Department of Molecular Genetics, Weizmann Institute of Science
contact email	emmanuel.amzallag@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076622

PRIDE project URI

• PRIDE
  1. PXD076622
     1. Label: PRIDE project
     2. Name: Ubiquitination regulates granulostasis and DRiP accumulation in SGs under heat stress via the E3 ligase MKRN2