PXD076445

PXD076445 is an original dataset announced via ProteomeXchange.

Title	ZO-1 phosphorylation upon ERK activation
Description	This dataset was generated to identify phosphorylation sites of ZO-1 regulated by ERK signaling. Cells were treated with TPA alone or with TPA in combination with the MEK inhibitor PD0325901, followed by phosphopeptide enrichment and LC–MS/MS analysis. TPA activates multiple kinases including ERK, whereas PD0325901 selectively inhibits MEK, the upstream activator of ERK. Thus, ERK activity is specifically suppressed in the co-treated condition. Phosphorylation sites detected in the TPA-treated samples but not in the TPA + PD0325901-treated samples are considered to be associated with ERK-dependent phosphorylation of ZO-1.
HostingRepository	PRIDE
AnnounceDate	2026-04-17
AnnouncementXML	Submission_2026-04-16_17:10:25.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sayuki Hirano
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: NEWT:9615;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-01 01:35:47	ID requested
⏵ 1	2026-04-16 17:10:26	announced

Dataset with its publication pending

submitter keyword: ERK,ZO-1, phosphorylation

Kazuhiro Aoki
contact affiliation	Laboratory of Quantitative Biology, Department of Gene Mechanisms, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Japan
contact email	aoki.kazuhiro.6v@kyoto-u.ac.jp
lab head
Sayuki Hirano
contact affiliation	Laboratory of Quantitative Biology, Department of Gene Mechanisms, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
contact email	hirano.sayuki.2k@kyoto-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076445

PRIDE project URI

• PRIDE
  1. PXD076445
     1. Label: PRIDE project
     2. Name: ZO-1 phosphorylation upon ERK activation