PXD076429

PXD076429 is an original dataset announced via ProteomeXchange.

Title	Identification of Transcriptional Repressors Recruited by Histone H3 Lysine 18 Lactylation in Nucleus Pulposus Cells via Affinity Purification-Mass Spectrometry
Description	This project aims to identify transcriptional repressors specifically recruited by histone H3 lysine 18 lactylation (H3K18la) in the nucleus pulposus cell context. Nucleus pulposus cells were cultured under lactic acid conditions to induce lactylation. Proteins interacting with H3K18la were immunoprecipitated using a specific H3K18la antibody, with parallel control pulldowns performed using normal IgG. The co-precipitated proteins from three biological replicates per condition were then analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The comparative IP-MS analysis between the H3K18la-IP and IgG control groups is designed to screen for putative transcriptional repressors that are selectively enriched by the H3K18la epigenetic mark.
HostingRepository	iProX
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-03-31_19:20:49.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yiming Ma
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-31 19:20:15	ID requested
⏵ 1	2026-03-31 19:20:49	announced

Dataset with its publication pending

submitter keyword: lactylation, nucleus pulposus cells

Zhange Yu
contact affiliation	the First Affiliated Hospital of Harbin Medical University
contact email	zhangeyu@hrbmu.edu.cn
lab head
Yiming Ma
contact affiliation	the First Affiliated Hospital of Harbin Medical University
contact email	1768372960@qq.com
dataset submitter

iProX dataset URI