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PXD076349
PXD076349 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PBS- and urea-based dual extraction of Bombyx mori cocoon coat proteins: comparative analysis of yield, bioactivity and proteomic differences |
| Description | The silkworm cocoon coat is a crucial natural barrier during the pupal stage, protecting the insect from patho-gens and environmental stress and containing silk proteins with notable antioxidant and antimicrobial activities. In this study, water-soluble proteins were extracted from the outer cocoon layer of the Bombyx mori N4 strain using phosphate-buffered saline (PBS) and 8 M urea, and the two buffer systems were compared in terms of protein yield, bioactivity, and proteomic composition. Urea-based extraction markedly increased the total protein yield, whereas the PBS extract showed stronger radical-scavenging capacity and more pronounced inhibition of fungal growth. Proteomic analysis further revealed clear differences between the two methods in sericin-to-fibroin ratios, recovery of defense-related proteins, and extraction efficiency for membrane-associated proteins. These results provide a ba-sis for the functional exploitation of cocoon coat proteins and for selecting appropriate extraction strategies ac-cording to different research or application goals. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-31_01:09:50.197.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hao Tian |
| SpeciesList | scientific name: Bombyx mori densovirus; NCBI TaxID: 10809; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-31 01:08:51 | ID requested | |
| ⏵ 1 | 2026-03-31 01:09:51 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Bombyx mori, cocoon coat proteins, urea extraction, proteomics, antioxidant activity, antimicrobial activity |
Contact List
| Chun Liu | |
|---|---|
| contact affiliation | State Key Laboratory of Resource Insects, SouthWestern University |
| contact email | mlliuchun@163.com |
| lab head | |
| Hao Tian | |
| contact affiliation | State Key Laboratory of Resource Insects, SouthWestern University |
| contact email | th1330193124@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
