PXD076279

PXD076279 is an original dataset announced via ProteomeXchange.

Title	Charting endocrine progenitors across species and organs
Description	Hormone-producing cells in the pancreas and intestine regulate whole-body metabolism and are key targets for causal therapies for diabetes and obesity. A deeper understanding of endocrine lineage formation (endocrinogenesis) in these organs is essential to elucidate disease mechanisms and develop improved therapies. Here, we performed cross-species (mouse-human) and cross-system (in vivo-in vitro) comparisons of pancreatic endocrine progenitors (EPs), complemented by cross-organ (pancreas-intestine) analyses, using single-cell transcriptomics and epigenomics integrated with bulk proteomics. We uncovered shared and distinct gene regulatory networks (GRNs) and cell-cell communication patterns during lineage allocation across species, systems, and organs. Additionally, we identified cardiometabolic risk-associated genes restricted to embryonic pancreatic EPs but largely absent in the adult pancreas, implicating developmental origins of disease. Furthermore, our integrated, multi-source EP profiling resolved the transcriptional programs underlying off-target enterochromaffin cell formation during in vitro differentiation toward human islet cells. Finally, we revealed conserved, dynamic shifts in programs governing the cell cycle, mRNA translation, and cytoskeletal organization during endocrinogenesis. Collectively, this multi-source EP profiling establishes a resource that charts the molecular landscape of endocrinogenesis and informs therapies for metabolic disease.
HostingRepository	PRIDE
AnnounceDate	2026-08-25
AnnouncementXML	Submission_2026-08-25_00:59:57.786.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Krahmer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 17:44:22	ID requested
⏵ 1	2026-08-25 00:59:58	announced

Dataset with its publication pending

submitter keyword: Diabetes, Ngn3,Pancreas, Proteomics, endocrine progenitor, β-cells

Natalie Krahmer
contact affiliation	Emmy-Noether Research Group Cellular Proteomics and Metabolic Signaling Institute for Diabetes and Obesity Helmholtz Diabetes Center Germany
contact email	natalie.krahmer@helmholtz-muenchen.de
lab head
Natalie Krahmer
contact affiliation	Helmholtz Center Munich
contact email	natalie.krahmer@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD076279

PRIDE project URI

• PRIDE
  1. PXD076279
     1. Label: PRIDE project
     2. Name: Charting endocrine progenitors across species and organs